A gateway to environmental signaling

e.hormone 2002 brochure

The Cutting Edge of Endocrine Disrupter Research
2nd Annual Symposium on the Environment and Hormones
October 15-18, 2000

  • Poster Abstracts
  • Conference Program

1. A Comparison of Illness Rates Between Children Exposed to Agricultural Pesticides and Non-Agricultural Children in Sonora, Mexico, Elizabeth Guillette, Ph.D., Department of Anthropology, University of Florida, Gainesville, Fl 32611, guillette@zoo.ufl.edu, Phone: 352-375-5929; fax: 352-392-6929

2. Detection of Several Endocrine Disruptors in Human Umbilical Cords and Sord Serum in Japan. Mori C1,2, Sakurai K 1, Iguchi T 2,3, 1Department of Anatomy, School of Medicine, Chiba University, 2Core Research for Evolutional Science and Technology CREST, 3Okazaki National Research Institutes, e-mail: mori@med.m.chiba-u.ac.jp, Phone: (81)-43-2262017 Fax: (81)-43-2262017

3. Administration of Bisphenol A During Pregnancy Results in Direct Fetal Exposure. K. Uchida 1, 2, 3, A. Suzuki 3, 4, D. Buchanan1,3, H. Watanabe1,3, Y. Kobayashi 5, J. Suzuki 6, K. Asaoka 6, C. Mori7. and T. Iguchi 1, 2, 3, 4, 1Center for Integrative Bioscience, Okazaki National Research Institutes, Okazaki, Aichi; 2Department of Molecular Biomechanics, Graduate University of Advanced Studies, 3CREST, JST, Tokyo; 4Graduate School of Integrated Science, Yokohama City University, Yokohama; 5Technical Laboratory, Analysis Center Corporation, Tokyo; 6 Primate Research Institute, Kyoto University, Inuyama, Aichi; 7Department of Anatomy, School of Medicine, Chiba University, Chiba, Japan. e-mail: taisen@nibb.ac.jp, Tel: +81-564-55-7525, Fax: +81-564-55-7526

4. Fetal Exposure to Phytoestrogens in Monkeys, Sakurai K1, Adachi T2, Shibayama T2, Asaoka K 3, Iguchi T2,4, Mori C1,2, 1Department of Anatomy, School of Medicine, Chiba University, 2Core Research for Evolutional Science and Technology CREST, 3Primate Research Institute Kyoto University, 4Center for Integrated Bioscience, Okazaki National Research Institutes, sakurai@med.m.chiba-u.ac.jp, Phone: +81-43-226-2017, Fax: +81-43-226-2018

5. Amphibians as Sentinels for Assessing the Effects of Endocrine Disruptors in the Environment. T.R. Barbeau and L.J. Guillette, Department of Zoology, University of Florida, Gainesville FL, tbarbeau@zoo.ufl.edu, Ph (352)392-1107, Fax (352)392-3704

6. Plasma Thryoxine (T4) Levels in Juvenille Alligators Collected from Lake Okeechobee and the Northern Everglades, Mark P. Gunderson, Dieldrich S. Bermudez, Teresa A. Bryan, Thea M. Edwards, Matthew R. Milnes, Allan R.Woodward, Louis J. Guillette, Department of Zoology, University of Florida, P.O. Box 118525, Gainesville, Fl. 32611, e-mail: mgunderson@zoo.ufl.edu, telephone: 352-392-1098

7. Acetochlor Alters Thyroid Hormone Receptor Gene Expression in Amphibian Tadpole Tails Without Modulating the Thyroid Axis, Nik Veldhoen1, Graham Van Aggelen2 and Caren Helbing1, 1Department of Biochemistry and Microbiology, P.O. Box 3055, University of Victoria, Victoria, B.C. Canada V8W 3P6, 2Environmental Toxicology Section, Pacific Environmental Science Centre, 2645 Dollarton Highway, North Vancouver, B.C. Canada V7H 1V2, E-mail: chelbing@uvic.ca, Phone: (250)721-6146, Fax: (250)721-8855

8. Shell Thickness of Alligator Eggs From Contaminated and Reference Lakes, Teresa Bryan, Matthew R. Milnes, and Louis J. Guillette, Jr., Department of Zoology, University of Florida, Gainesville, FL 32611, kuba@ufl.edu, (352) 392-1098, fax (352) 392-3704

9. Altered Neonatal Development and Endocrine Function in American Alligators (Alligator mississippiensis) from a Contaminated Lake in Florida, Matthew R. Milnes, Teresa A. Bryan, Mark P. Gunderson, Louis J. Guillette, Jr., Department of Zoology, University of Florida, 223 Bartram Hall, Gainesville, FL 326011, Email: mrmilnes@zoo.ufl.edu, Phone: (352) 392-1098, Fax: (352) 392-3704

10. Effects of Endocrine-Disrupting Contaminants on American Alligator (Alligator mississipiensis) Spleen and Thymus Morphology, Dieldrich S. Bermudez, Andrew A. Rooney, Louis J. Guillette, Jr., Department of Zoology, University of Florida, Gainesville, FL 32611, dbermudez@zoo.ufl.edu, (352) 392-1098, fax (352) 392-3704

11. Gonadotropin Induction of Testosterone Synthesis in Alligators from Contaminated and Uncontaminated Environments., Thea M. Edwards, Mark P. Gunderson, Edward F. Orlando, and Louis J. Guillette, Jr., University of Florida, Department of Zoology Gainesville, FL 32611-8525., Email: tedwards@zoo.ufl.edu, Phone: (352) 392-1098, FAX: (352) 392-3704

12. Effects of Exposure to Paper Mill Effluents in Lepomis macrochirus from Elevenmile Creek, Florida, JR Burse1, AO Cheek2, and HL Bart Jr.1, 1 Tulane University Ecology and Evolutionary Biology New Orleans,LA 70118, 2 Southeastern Louisiana University Hammond,LA 70402, jburse@tulane.edu, (504)8628000x1558, fax(504)8628706

13. Effects of Paper Mill Effluent on a Population of Eastern Mosquitofish, Gambusia holbrooki, Heather B. McNatt1, Robert A. Angus1, and W. Mike Howell2, 1Biology Department, University of Alabama at Birmingham, Birmingham, AL 35294, 2Biology Department, Samford University, Birmingham, AL 35229, e-mail: myrmekiaphila@juno.com, Phone: (205) 824-0400, Fax: (205) 975-6097

14. Androstenedione is Present in a River Containing Paper Mill Effluent and Masculinized Fish, Ronald Jenkins1, Robert Angus2, Heather McNatt2, W. Mike Howell1, Jon A. Kemppainen3, Marion Kirk2, and Elizabeth Wilson3, 1Dept. of Biology, Samford University, Birmingham, AL 35229, 2Dept. of Biology, Univ. of Alabama at Birmingham, Birmingham, AL 35229, 3Laboratories for Reproductive Biology and the Dept. of Pediatrics, Biochemistry and Biophysics, University of North Carolina, Chapel Hill, NC 27599, rljenkin@samford.edu, Phone (205) 726-2947, FAX (205) 726-2479

15. Effects of Masculinization on the Reproductive Physiology of Female Mosquitofish Gambusia affinis., Jason P. Stanko and Robert A. Angus, University of Alabama at Birmingham, Dept. of Biology, Birmingham, AL., jpstanko@uab.edu, T 205-934-4799/4282, F 205-975-6097

16. Sexual Characteristics are Altered by 17b-estradiol and 4-tert-octylphenol in the Guppy (Poecilia reticulata)., Toft, Gunnar and Baatrup, Erik., Institute of Biological Sciences, University of Aarhus, Building 135, DK-8000 Aarhus C, Denmark, e-mail gunnar.toft@biology.au.dk, Phone +45-8942-2720, Fax +45-8612-5175, Temporary address (until 12/20/00), Department of Zoology, 223 Bartram Hall, PO box 118525, University of Florida, Gainesville, FL 32611, e-mail gtoft@zoo.ufl.edu, Phone (352) 392-1098, Fax (352) 392-3704

17. Temporal and Quantitative Changes in Sexual Reproductive Cycling of the Cladoceran Daphnia magna by a Juvenile Hormone Analogue, Olmstead, A.W. and LeBlanc, G.A., North Carolina State University, Raleigh, NC., Gal@ncsu.edu, T 919-515-7404, F 919-515-7169

18. Biotransformation and Disposition of Testosterone in the American Mud Snail, Ilyanassa obsoleta., Gooding, M.P. and LeBlanc, G.A.North Carolina State University, Raleigh, N.C., GA_LeBlanc@ncsu.edu, T 919-515-7404, F 919-515-7169

19. Uptake of Dissolved Estrone by Scleractinian Corals, A.M. Tarrant1, M.J. Atkinson2, and S. Atkinson3, 1 Department of Oceanography; University of Hawaii at Manoa; Honolulu, HI., 2 Hawaii Institute of Marine Biology; University of Hawaii at Manoa, Kaneohe, HI., 3 Alaska Sealife Center, University of Alaska, Seward, AK., Phone: (808) 956-6050; Fax: (808) 956-9516; Email: atarrant@soest.hawaii.edu

20. Evidence of a Nuclear Receptor in Two Species of Scleractinian Corals, A.M. Tarrant1, T.C. Chiang2, J.A. McLachlan2, S. Li2, 1Department of Oceanography; University of Hawaii at Manoa; Honolulu, HI., 2Center for Bioenvironmental Research, Tulane University; New Orleans, LA., Email: atarrant@soest.hawaii.edu, Phone: (808) 956-6050; Fax: (808) 956-9516

21. Estrogenic and Antiestrogenic Activity of Legumes and Other Plants Containing Phytoestrogens, Stephen M. BouŽ1, Thomas E. Wiese2, Carol H. Carter-Wienjtes1and Thomas E. Cleveland1, 1Southern Regional Research Center, USDA, New Orleans, LA 70124, 2College of Pharmacy, Xavier University of Louisiana, New Orleans, LA 70118, Email: sboue@nola.srrc.usda.gov, Telephone: 504-286-4346, Fax: 504-286-4419

22. The Mycoestrogen Zearalenone Affects the Proliferation of Mouse Primordial Germ Cells in Culture and Activates the Steel Gene Promoter via an AP-1 Response Element., G.H.G. Behrens, F.G. Klinger, M. Pesce, W. Eskild, T. Grotmol, T. B. Haugen, and M. De FeliciNational Hospital (GHGB, TBH), Norway; University of Rome "Tor Vergata"(FGK, MP, MDF), Italy; Institute for Epidemiological Cancer Research (TG), Norway; University of Oslo (WE), Norway., e-mail: g.h.g.behrens@klinmed.uio.no; phone: +47 23074962, fax: +47 23072940

23. Preferential Interaction of PAH-related Compounds with the beta Isoform of the Estrogen Receptor., K C Fertuck1, H Sikka2, J B Matthews1 and T R Zacharewski1., 1Department of Biochemistry and National Food Safety and Toxicology Center, Michigan State University, East Lansing, MI, USA., 2Division of Environmental Toxicology & Chemicals, SUNY-Buffalo, NY., fertuckk@pilot.msu.edu, 517-353-1944, fax 517-353-9334

24. Effects of Polybrominated Diphenyl Ethers (PBDEs) on Neonatal Rat Ventral Prostate Grown in Culture, K.A. Thayer1, G. Marsh2, A. Bergman2, R. Dahiya3, G. Cunha1,1 Department of Anatomy, University of California-San Francisco, San Francisco, California, USA, 2 Department of Environmental Chemistry, Stockholm University, Stockholm, Sweden, 3 Department of Urology, Veterans Affairs Medical Center, San Francisco, California, USA., thayer@itsa.ucsf.edu, Ph: 415-476-4510, Fax: 415-502-2270

25. The Effects of DDT and its Metabolites on AP-1 Activity: ER Dependent and Independent Mechanisms, Daniel E. Frigo, Matthew E. Burow, Kamron A. Mitchell, Steven Elliott, and John A. McLachlan., Molecular and Cellular Biology Program and Environmental Endocrinology Project at Tulane-Xavier Center for Bioenvironmental Research, New Orleans, LA 70112, E-mail: dfrigo@tulane.edu, Tel (504) 988-6623; Fax (504) 988-6215

26. Cross-Talk Between PI3K-AKT and the Estrogen Receptor, Bich Duong, Matthew Burow, Daniel Frigo, Steven Elliott, Christopher Weldon, Bridgette Collins-Burow, Jawed Alam, Barbera Beckman, and John McLachlan, Molecular and Cellular Biology Program and Environmental Endocrinology Project at the Tulane-Xavier Center for Bioenvironmental Research, New Orleans, LA 70112, e-mail: bduong@tulane.edu Phone (504) 988-6623; Fax (504) 988-6215

27. Regulation of Human Ah receptor Signaling by Chaperone Proteins in a Yeast Model System., Charles Miller1 and Marc Cox2, Environmental Health Sciences Dept. 1 and Molecular and Cellular Biology Program1,2 Tulane University Medical Center, New Orleans, LA 70112, rellim@tulane.edu, (504)585-6942

28. Response Element Sequence Determines Xenoestrogen Activation of Estrogen Regulated Reporter Genes, Lawanda Miller-Schief1, Suzanne Nehls2 and Thomas E. Wiese2, 1Interdisciplinary Graduate Program in Molecular and Cellular Biology and the 2Department of Environmental Health Sciences, CBR at Tulane-Xavier Universities, twiese@tulane.edu, (504) 988-6376

29. Xenoestrogen Activity in Human and Trout Estrogen Receptor Reporter Assays: Species Specific Agonist and Antagonist Effects, Suzanne Nehls1 and Thomas E. Wiese1,2, 1Division of Basic Pharmaceutical Sciences, Xavier University College of Pharmacy; 2Department of Environmental Health Sciences, Tulane University School of Public Health and Tropical Medicine, New Orleans, LA, USA, twiese@tulane.edu, (504) 488-2243

30. Estrogenic Activity of Novel Synthetic Non-Steroidal Chemicals:Molecular Modeling and Fluorescence Polarization Studies., Rajendram V Rajnarayanan, Matthewos Eshete, Georgi Nikov and William L. Alworth*, Department of Chemistry, Tulane University, New Orleans, LA 70118, Email: alworth@tulane.edu

31. Homology Modeling of the Estrogen Receptor Subtype b (ERb), the Androgen Receptor (AR), and the Glucocorticoid Receptor (GR): Investigation of Mutational Effects on Ligand Binding., Robert Kirk DeLisle, Seong-Jae Yu, Anil C. Nair, Christopher Mills, and William J. Welsh, Department of Chemistry and Center for Molecular Electronics, University of Missouri-St. Louis, St. Louis, MO 63121 (USA)., E-mail WWELSH@UMSL.EDU, phone (314-516-5318), Fax (314-516-5342)

32. Phytochemical Signaling and Symbiosis: Potential Threats from Endocrine Disrupting Chemicals, Fox, J., Starcevic, M., Kow, K., McLachlan, J., Molecular and Cellular Biology Program and Environmental Endocrinology Project at the Tulane-Xavier Center for Bioenvironmental Research, New Orleans LA 70112, email: jfox3@mailhost.tcs.tulane.edu, phone (504)988-6623, fax (504)988-6215

33. Use of a Fiber Optic Biosensor to Demonstrate the Presence of an Estrogen Mimicking Substance in Pond Water Hosting Malformed Frogs., Judith l. Erb,1 Eric A.E. Garber,2 Eric M. Priuska,1 James L. Wittliff,3 and James G. Downward1, 1IA Inc. and Threefold Sensors, Ann Arbor, MI, 48106., 2USDA-ARS Biosciences Research Lab., Fargo, ND 58105, 3Univ. Of Louisville, KY 40292, Email: tfs-jerb@ic.net, Ph: (734) 995-9338 x20, FAX: (734) 995-6869

34. Connecting Ecological Data with Epidemiological Maps: Speed Dialing Instructions, J. Lisa Jorgenson, Consultant, International Water Specialist, (World Bank, Global Environment Fund, World Resource Report, IntlÕ Union for the Conservation of Nature), 2335 California Street NW, Washington, D.C. 20008, e-mail: ljorgenson@igc.org, TEL: 202-462-1929, FAX: 202-462-5703

35. Endocrine Disruptors Research Program at the US Environmental Protection Agency, Elaine Z. Francis, Office of Research and Development, USEPA, Washington, DC, e-mail: francis.elaine@epa.gov, Phone: (202) 564-6789, Fax: (202) 565-2444

36. Sperm concentration in young men from Nordic-Baltic countries Ð inversely related to testicular cancer incidence ?, T.B. Haugen1*, A. Giwercman2, T. Grotmol3, ¯. Magnus1, V. Matulevicius4, I. Nermoen1, M. Punab5, J. Richthoff2, and B. Zilaitiene4, 1Andrology Laboratory, Department of Gynecology and Obstetrics, National Hospital, Oslo, Norway; 2Department of Urology, Malmš University Hospital, Lund University, Malmš, Sweden; 3 The Cancer Registry of Norway, Montebello, Oslo, Norway; 4Institute of Endocrinology, Kaunas University of Medicine, Kaunas, Lithuania; 5Clinics of Surgery, Tartu University Clinics, Tartu, Estonia, e-mail: t.b.haugen@rh.uio.no; phone: + 47 23 07 26 31; fax: + 47 23 29 40

Technical Demonstrations at the "Cutting Edge": The Latest Techniques for Endocrine Disruptor Research

1. Development of Nonisotopic High-Throughput Screening Assay for Estrogen and Androgen Receptor Ligands, Yoshihiro Soya, Shigeaki Nishii, Kazuhiro Matsui,Takuya Ishibashi and Yoshihisa Kawamura, Tsuruga Institute of Biotechnology Toyobo Co.,Ltd, yoshihiro_soya@bio.toyobo.co.jp

2. Demonstration of Fiber Optic Sensor for Recording Real Time Binding Between Biomolecules, Judith L. Erb and James G. Downward, IA Inc. and Threefold Sensors, Ann Arbor, MI, 48106., Email: tfs-jerb@ic.net, Ph: (734) 995-9338 x20, FAX: (734) 995-6869

3. Recombinant Human Glucocorticoid Receptor in a Fluorescence Polarization-Based Ligand Binding Assay, C. Halbleib, B. Mei, M. Ozers, E.B. Thompson*, W. Checovich, and R.G. Lowery, PanVera Corporation, 545 Science Drive; Madison, WI 53711, *Human Biological Chemistry and Genetics; University of Texas Medical Branch; Galveston, TX 77555-0645, caleh@panvera.com, Phone: (608) 233-9450 x 247, Fax: (608) 233-0857

4. A Sensitive Method for Monitoring Gene Responses to Environmental Estrogens, E Thomas-Jones, C Morris, G Thomas, J Cryer, I Weeks, S Woodhead & P Kille*, Molecular Light Technology Research Limited, 5 Chiltern Close, Cardiff CF14 5DL, *School of Biosciences, Cardiff University, PO Box 911, Cardiff CF1 3US., emma.thomas-jones@mltresearch.com, Tel: +44(0)29 2074 7033, Fax: +44(0)29 2074 7118

5. Endocrine Disruptor Knowledge Base (EDKB) Database and Software, Weida Tong2, Megan Cao2, Steve Harris2, H. Fang2, Qian Xie2, Huixiao Hong2, R. Perkins2, W. Branham1, D. Sheehan1, 1FDA National Center for Toxicological Research, 2R.O.W. Sciences, Jefferson, AR, WTong@nctr.fda.gov

6. Opening the Door for Estrogens: A Hands on Demonstration of Computational Techniques, Thomas C.Bishop and Andrew Hall, Center for Bioenvironmental Research, Tulane University, Dept. of Environmental Health Sciences, Xavier University, College of Basic Pharmaceutical Sciences, bishop@tulane.edu, phone: 504-988-6203, fax: 504-585-6428

Monday, October 16, 2000


Chair: VALERIE WILSON, Center for Bioenvironmental Research, Tulane and Xavier Universities, Louisiana.

NIELS SKAKKEBAECK, Department of Growth and Reproduction, GR Rigshospitalet, Denmark, "Testicular dysgenesis syndrome."

BERNARD JEGOU, Universite de Rennes, France, Male Reproduction and the Environment

CHERYL WALKER, College of Pharmacy, University of Texas, "Molecular mechanisms of aberrant steroid hormone response in uterine leiomyoma."

SHUAN FANG LI, Center for Bioenvironmental Research, Tulane and Xavier Universities, Louisiana, "Estrogen associated genes in uterine dysfunction."

MYRON MOOREHEAD, The Women's Health Institute, Louisiana, "Uterine fibroids: a treatable disease."


Chair: ANN CHEEK, Department of Biological Sciences, Southeastern Louisiana University.

MARIA CHRISTINA FOSSI, Department of Environmental Biology, Universities of Siena and Messina, Italy, "Biomarkers as diagnostic and prognostic tools for assessing effects of endocrine disrupters among top predators in the mediterranean ecosystem."

EVA OBERDOESTER, Department of Environmental Toxicology, Clemson University, South Carolina, "Peptide hormones vs. steroid hormones: case studies from snail and turtle populations."

DOUG MEFFERT, Center for Bioenvironmental Research, Tulane and Xavier Universities, Louisiana, "Comprehensive integrated ecosystem and human risk assessment for BPA - problem formulation."



Tuesday, October 17, 2000



Chair: TAISEN IGUCHI, Center for Bio-environmental Research, National Basic Biology Institute, Japan.

JOHN COUSE, Laboratory of Reproductive and Developmental Toxicology, National Institute of Environmental Health Sciences, North Carolina, "Sexual development in the ERKO, BERKO and double knock out mice - what does it tell us about estrogen?"

TAKESHI KURITA, Department of Anatomy, University of California, San Francisco, "P63 and genital tract morphogenesis."

GEN YAMADA, Center for Animal Resources and Development, Kumamato University, Japan, "Genes involved in the development of external genitalia: lessons for hypospadias."

HOLLY INGRAHAM, Department of Physiology and OB-GYN, University of California, San Francisco, "Orphan nuclear receptors in endocrine organ development - will they be adopted by ligands?"


Chair: LOU GUILLETTE, Department of Zoology, University of Florida

JERRY LeBLANC, Department of Toxicology, North Carolina State University, Invertebrate Endocrine Responses to Environmental Signaling

IAN CALLARD, Department of Biology, Boston University, C. Elegans as a Model for Endocrine Change



Chair: JOHN McLACHLAN, Center for Bioenvironmental Research, Tulane and Xavier Universities

SHELDON KRIMSKY, Tufts University, The Scientific and Social Roots of the Endocrine Disrupter Hypothesis.




BANQUET: Dinner speakers on Hormonal Histories and “Herstories”


Wednesday, October 18, 2000



Chair: WILLIAM TOSCANO, Division of Environmental & Occupational Health, The University of Minnesota School of Public Health

WENCHAO SONG, Center for Experimental Therapeutics and Department of Pharmacology, University of Pennsylvania School of Medicine, Modulation of Estrogen Activity in the Reproductive System by Estrogen Sulfotransferase Ð A Study Using Estrogen Sulfotransferase Gene Knockout Mice

SARASWATI SUKUMAR, Breast Cancer Program, Johns Hopkins University School of Medicine, Methylation-Mediated Silencing of Gene Expression in Breast Cancer

ROBERT BRUEGGEMEIR, College of Pharmacy, The Ohio State University, Effects of phytoestrogens and synthetic analogs on aromatase and estrogen biosynthesis.

JAN-AKE GUSTAFSSON, Departments of Medical Nutrition and Biosciences, Karolinska Institute, Sweden, Estrogen Receptor beta (ER beta) Ð A Paradigm Shift in Our View on Estrogen Signaling and Endocrine Disruption


Closing Remarks - JOHN McLACHLAN

Disclosure Statement

It is the policy of the Center for Continuing Education at Tulane University Health Sciences Center to ensure balance, independence, objectivity and scientific rigor in all its educational programs. All faculty participating in these programs are expected to disclose to the program audiences any real or apparent conflict of interest related to the content of their presentations. This information pertains to relationships with pharmaceutical companies, biomedical device manufacturers or other corporations whose products or services are related to the subject matter of the presentation topic or products in the research and development phase.