e.hormone environmental signaling epigenetics lead in the environmental

e.hormone 2002 brochure

The Cutting Edge of Endocrine Disrupter Research
5th Annual Symposium on the Environment and Hormones
October 16-18, 2003
Hosted by: Tulane/Xavier Center for Bioenvironmental Research

  • General Information
  • Poster Abstracts
  • Conference Program
General Information

We contend that the phenomenon known as endocrine disruption is a manifestation of a larger biological issue ­ that is, the complex signaling networks that exist within and between organisms. These signaling networks can be maintained or perturbed by environmental factors. At e.hormone we intend to develop the tools for a new science of environmental signaling and introduce participants to the Environmental Signaling Network (an NSF-sponsored activity).

Themes of the meeting are:
1) environmental systems biology
2) fetal and childhood patterning, and
3) hormones and pharmaceuticals in water.

Poster Abstracts

In vitro Mechanisms and Signals

1. Inhibition or Induction of Apoptosis in Human Breast Cancer Cells via Estrogen Receptor Mediated Action, Sujung Lee, Jae-Ho Shin, Taesung Kim, Hyunju Moon, Ilhyun Kang, Inyoung Kim, Jiyoung Oh, and Soon -Young Han

2. Regulation of Cell Proliferation by Polycyclic Aromatic Hydrocarbons in MCF-7.BOS Breast Cancer Cells, M. Plí_ková, J. Vondrá_ek, M. Machala

3. Rapid, G-Protein Dependent Signaling Events Triggered by 17-b Estradiol in Myometrial and Leiomyoma Cells, Erica N. Simpson, Karla Johanning, and John A. McLachlan

4. Detection of HMGA 1 and 2 in Uterine Leiomyoma Cells and Tissues, M. M. Martin, T.C. Chiang, C.D. Swartz, D. Dixon, and J.A. McLachlan

5. Antihormonal Effects of the Soybean Phytoalexin Glyceollin, Stephen M. Boué, Matthew E. Burow, Thomas E. Wiese, Betty Y. Shih, Carol H. Carter-Wientjes, Virgilio S. Morales and Thomas E. Cleveland

6. Evaluation of the Estrogen, Androgen and Glucocorticoid Activity of Dietary Supplements Commonly used to Treat Postmenopausal Symptoms, Nicole Lee, Michelle Lang, Huiming Li, Stephen M. Boué, Thomas E. Wiese

7. Enantiomer Selective Hormone Activity of the Pesticide Fipronil: Antiandrogen and Antiprogesterone Activity, Thomas E. Wiese, Suzanne Nehls, Huiming Li, Cheryl K. Stevens, A. Wayne Garrison

8. Long-Term Exposure to b-Hexachlorocyclohexane Promotes Transformation and Invasiveness of MCF7 Human Beast Cancer Cells, Enmin Zou and Fumio Matsumura

in vivo Mechanisms and Signals

9. Isomer and Tissue-Specific Activity of Dichlorodyphenyl-trichloroethane (DDT) with Estrogen Receptor in Adult and Suckling Estrogen Reporter Mice, D. Di Lorenzo, M.L. Penza, E. Bonetti, G. Biasiotto, P. Apostoli, M. Raviscioni, P. Ciana, A. Maggi and , R. Villa

10. Fetal exposure to Low Doses of Bisphenol A alters steroid receptor levels and DNA synthetic activity in the uterus of the adult mouse, Perinaaz R. Wadia, Caroline M. Markey, Beverly S. Rubin, Carlos Sonnenschein and Ana M. Soto

11. Age-related Changes of Reproductive Functions in Female Rats Given Bisphenol-A Neonatally, Ishibashi, T. Ohta, Y., Kato, H, Iguchi, T.

12. Estrogen-Independent Activation of ErbBs Signaling and Estrogen Receptor in the Mouse Vagina Exposed Neonatally to Diethylstilbestrol, Shinichi Miyagawa, Yoshinao Katsu, Hajime Watanabe and Taisen Iguchi

13. Developmental Diethylstilbestrol Exposure Reveals Novel Genetic Pathways of Uterine Cell Fate Determination, Wei-Wei Huang, Yan Yin, Qun Bi, Tung-Chin Chiang, Jussi Vuoristo, Darwin J. Prockop, John A. McLachlan and Liang Ma

14. Analysis of Effect of Diethylstilbestrol (DES) on Mouse Male Reproductive Organs Using Transplantation Technique, Microarray and RLGS Method, Hideki Fukata, Kyu-Bom Koh, Kohji Sato, Koji Yamazaki and Chisato Mori

Wildlife Exposure and Mechanisms

15. Using a Macroarray to Identify Estrogen Responsive Genes During Early Development of a Native Amphibian, Rana pipiens, Natacha Gallant, Vance Trudeau, David Lean, and Caren Helbing

16. Gene expression patterns in juvenile American alligators (Alligator mississippiensis) exposed to environmental contaminants, Satomi Kohno, Yoshina Katsu, Teresa Bryant, Dieldrich Bermudez, Mark Gunderson, Brandon Moore, Taisen Iguchi and Louis J. Guillette Jr.

17. Characterization of ER and AR distribution by Immunocytochemistry in the Alligator (A. mississippiensis) Thyroid during different Life Stages, Jeremy Skotko, Dieldrich Bermudez, Allen Woodward and Louis J. Guillette, Jr.

18. Nucleotide Sequence of an Estrogen Receptor Gene from Lepisosteidae, Ferrara, AM, Fontenot, QC, Chiang, TC, Baldwin, K, Kamath, M, Kissane, K, Melink, L, Johanning, KM and McLachlan, JA

19. Seasonal Variation of Plasma Thyroxine Concentrations in Juvenile Alligators (Alligator mississippiensis) from three Florida Lakes, Dieldrich S. Bermudez, Teresa A. Bryant, Mark P. Gunderson, Matthew R. Milnes, Christopher Tubbs, Allen R. Woodward and Louis J. Guillette, Jr.

20. Phallic Size Variation in Yearling Alligators Exposed to Naturally Occurring Environmental Contaminants, Teresa Bryan, Mathew R. Milnes, Louis J. Guillette, Jr.

21. Temperature affects vitellogenesis in estrogen-treated male mosquitofish, Paul D. Melvin, III and Robert Angus

22. Effects of Dietary and Static Renewal Exposure of Androstenedione on Female Mosquitofish, Gambusia affinis, Stanko, JP, Dean, JR, Angus, RA

23. Altered Reproductive Biology of Mosquitofish (Gambusia holbrooki) Exposed to Paper Mill Effluent, E.F. Orlando, W.P. Davis, L.E. Gray, Jr., and L.J. Guillette, Jr.

24. Preliminary Observations of Atrazine-Induced Effects upon Gonadal Differentiation in Rivulus marmoratus, a Naturally Hermaphroditic Fish, William P. Davis, Geraldine M. Cripe, Arunthavarani Thiyagarajah, Edward F. Orlando, Brenna Doheny and Kenneth J. Bogel

25. Decreased Gonadal Steroidogenesis and Increased Plasma Insulin-Like Growth Factor-1 in Frogs Exposed to Ecologically-Relevant Doses of Aquatic Nitrates, Tamatha R. Barbeau and Louis J. Guillette, Dept. of Zoology, University of Florida

26. Long-term Exposure to UV-B Radiation and the Estrogenic Endocrine Disrupting Chemical 4-tert-octylphenol (4-t-OP) affects Development and Metamorphosis of Rana pipiens Tadpoles, Croteau, M.C., D.R.S. Lean and V.L. Trudeau.

27. The Effects of Nitrate on Amphibian Metamorphosis, Thea M. Edwards, Krista McCoy, Rebecca Emrich, Hilary Thompson, John Matt Thro, and Louis J. Guillette, Jr.

28. Antiecdysteroidal Activity of Juvenoid Hormones in a Crustacean, Xueyan Mu, Heather L. Hoy, and Gerald. A. LeBlanc

29. Coordinate Regulation of Hemoglobin Production and Reproduction in a Crustacean by the Hormone Methyl Farnesoate and Their Dysregulation by Insecticidal Insect Growth Regulators, Gerald A. LeBlanc, Allen W. Olmstead, Cynthia V. Rider, Heather L. Hoy, and Beth A. Wasilak

30. Seasonal and Tissue-Preferential Expression of Estrogen Receptor Family Gene in Gastropod, Thais Clavigera, Masaaki Kajiwara, Hitomi Enomoto, Kenichi Kato, Shingo Toda, Shigeru Takahashi, Takashi Miura and Yuji Takahashi

31. Imposex in a Pacific Coastal Area in Costa Rica, Gravel, P, Johanning, KM, McLachlan, JA; Vargas, JA and Oberdörster, E

32. Levels of Steroid Hormones in an Agricultural Watershed: A Case Study of the North Bosque River, E. Oberdörster, P. Gravel, B. North, F. Diggs, J.H. Easton

33. Initial Observations of Intersex from the South Platte and Arkansas River drainages in Colorado, John Woodling, Elena Lopez, Tammy Maldonado, David Norris, and Alan Vajda

Human Effects

34. Hormonal and Neurodevelopmental Effects of Prenatal Exposure to Thyroid Hormone Disruptors in a Farmworker Community, Brenda Eskenazi, Asa Bradman, Laura Fenster, Jonathan Chevrier, Dana Barr, Martha Harnly, Bob McLaughlin, Rusty Scalf, Eleanor A. Gladstone, Abbey Alkon Caroline Johnson and Marcy Warner

35. Dioxin activities in human samples as effect markers of organochlorine bioaccumulation, Eva C. Bonefeld-Jorgensen and Manhai Long

Environmental Signals:
Elimination, Identification and Education

36. An Approach to Solve the Problem of Endocrine Disrupting Plasticizer Migrated from Flexible PVC Materials, Jeongsoo Choi, Seung-Yeop Kwak

37. Development of a Biosensor for Estrogenic Compounds Using a Quartz Crystal Microbalance, Linda A. Luck, Kendra Carmon and Ruth Baltus

38. Validation Progress of the LUMI-CELLTM ER Recombinant Bioassay for Rapid Evaluation of Chemicals for Potential Estrogenic Activity, John D. Gordon, Andrew C. Chu, Michael D. Chu, Charlotte L. Taylor, Michael S. Denison and George C. Clark

39. Assessment of Xenoestrogen Recognition by Recombinant Human Estrogen Receptors (hER) Using Ligand Titration Arrays, Sarah A. Andres, Stefanie B. Bumpus, Irina A. Smolenkova, Andrei S. Smolenkov, Molly E. Drysen, Judith L. Erb and James L. Wittliff

40. Receptor-based Biosensor Responses Correlate with Fish Vitellogenin Production in Great Lakes Water Samples, Judith L. Erb, James G. Downward IV, Randy Lehr, Deborah L. Swackhamer, James L. Wittliff

41. A Multidisciplinary/Intercollegiate Collaborative Experience Using a Recombinant Yeast to Quantify Estrogenic Compounds in Wastewater, Joseph C Colosi, Arthur Kney, Holly J. Morris

42. Estrogen-degrading Cultures in Activated Sludge, Ching-Ping Yu, Yuechuan Yang, Kung-Hui Chu

43. In vivo Generation of Androgens from the Microbial Degradation of Progesterone, Ronald L. Jenkins, David Smith, Elizabeth M. Wilson, W. Mike Howell and Marione Nance

44. QSAR Models of the in vitro Estrogen Activity of Bisphenol A Analogs, K. P. Coleman, W. A. Toscano, Jr., T. E. Wiese

45. Development of a Structure Database of Endocrine Disruptors, Bascos, NA, Bloom, J, and Bishop, TC

Conference Program
wednesday, october 15, 2003
The Environmental Signaling Network Presents A Communication and Education Workshop (optional)
sponsored by the National Science Foundation at the Pere Marquette Hotel
9:00 - 1:00 pm Environmental Signaling 101: the Basics of Endocrine Disruption (ES 101)

ES 101 will provide a fundamental understanding of the principles underlying endocrine disruption. The goal of this workshop is to make complex issues understandable and useable. This day of activities is free; we hope to attract the interactive participation of curious, environmentally concerned teachers and students. Course instructors are leading researchers in their areas of inquiry as well as expert communicators. The course will be presented in three 50-minute modules followed by faculty-guided discussion/activity groups.

Instructors: John McLachlan (Tulane University), Lou Guillette (University of Florida, Gainesville), Cindy Gulledge (University of Louisville), Tyrone Hayes (University of California, Berkeley), William Toscano (University of Minnesota), Thomas Wiese (Xavier University)
1:00 - 2:00 pm lunch
2:00 - 4:00 pm ES 101 Curriculum Discussion and Development

This important feedback session will be devoted to critically analyzing ES 101. The goal is to refine course information and instruction and to create methods for curricular adaptation, dissemination, and implementation. ES 101 attendees are asked to participate.
6:00 - 9:00 pm e.hormone welcome reception (at the pere marquette hotel)
thursday, october 16, 2003
7:45 - 8:45 am continental breakfast
Opening Remarks and Introduction to the Environmental Signaling Network
9:00 - 9:30 am John McLachlan
Center for Bioenvironmental Research
A Systems Biology Approach to Environmental Signaling
9:30 - 12:40 pm Chair, William Toscano, Jr.
University of Minnesota
  George Stancel
University of Texas at Houston
  Chisato Mori
Chiba University, Japan
“Genomic and Post-Genomic Analysis of Endocrine Disruption.”
  Stephen Harris
University of Missouri
“Modeling of Regulatory Gene Networks in Mammalian Cells.”
  Philip Iannaccone
Northwestern University
"GLI Transcription Factors: Participation in Networks of Regulation."
Signaling in Gene Networks
1:40 - 5:30 pm Chair, Matthew Burow
Tulane University
  Elwood Linney
Duke University
“Transgenic Fish Approaches to Receptor Pathways"
  Angel Nadal
Miguel Hernández University, Spain
"New Insights in Xenoestrogen Actions: From Plasma Membrane to Nuclear Function"
  Masahiko Negishi
“Nuclear Receptor CAR as a New Estrogen Target: Linking Hormone Action and Metabolism”
  Shiuan Chen
City of Hope Beckman Research Institute
“Orphan Receptors, Environmental Ligands and Aromatase”
  Ganka Nikolova
University of California, Los Angeles
“WNT4 Inhibition of Steroid Biosynthesis”
evening poster session and banquet (at "old bank bldg," 210 baronne, across street from pere marquette)
Chair — Tom Wiese, Xavier University
friday, october 17, 2003
7:45 - 8:45 am continental breakfast (at the pere marquette hotel)
Developmental Patterning
9:00 - 6:30 pm Chair, Liang Ma
Tulane University
  DJP Barker
Southampton General Hospital, UK
“The Developmental Origins of Chronic Disease”
  David Sassoon
Mount Sinai School of Medicine
“Patterning Circuits Hijacked: Wnt Genes Under the Control of DES”
  Michaela Luconi
University of Florence, Italy
“Effects of Estrogens and Estrogen-like Compounds on Differentiation of Human Male External Genitalia"
  coffee break
  Pat Hunt
Case Western Reserve University
“Induction of meiotic aneuploidy in mice associated with inadvertent contamination by a hormonally active plasticizer: implications for humans”.
  Jeff Boyd
Memorial Sloan-Kettering Cancer Center
"Gene Silencing by Estrogen Receptor-Dependent Promoter Methylation."
  Louis Guillette
University of Florida
Workshop Session I – Developmental Estrogenization Syndrome (at hotel)
  Through this provocative and enlightening exercise we will seek to create a hypothetical model for developmental induction of adult health and disease.

Panelists will include, epidemiologists, molecular biologists, developmental systems biologists, consumers, clinicians, ecologists, and experimental biologists.
Workshop Session II – Environmental Signaling: An Intersection of the Arts, Humanities, & Science (at hotel)
  This workshop will address a two-fold issue:
  1. is environmental signaling a good metaphor in which to help art, science, and the humanities meet?
  2. what can such convergence create that can not be generated independently?
Panelists will present philosophic, personal, movement, & sculptural perspectives.
  free evening
saturday, october 18, 2003
7:45 - 8:45 am continental breakfast
Hormones and Pharmaceuticals in Water
9:00 - 2:00 pm Chairs, Doug Meffert and Glen Boyd
  Steve McDonald
Carollo Engineering
“Macro-engineering of water quality in the age of drugs and pharmaceuticals”
  Herbert Buxton
USGS Toxics Program
“Occurrence and Implication of Hormones and Pharmaceuticals in America's Streams and Waterways”
  Patrick Larkin
EcoArray, LLC
“Genomic Approaches to Biomarkers of Effect in Aquatic Species”
  James Witliff
University of Louisville
“Xenoestrogen Detection and Assessment with Biosensors and Gene Expression Profiling.”
  Xia Li
Tulane University
“Antibody Based Biosensor for Heavy Metals in the Environment”
Workshop III – The River Runs Through Us (with lunch)
  Posters and discussion with area high school teachers and students.
• What do we need to do and how should we do it?
• Is there an overall approach to determine impact of biologically active materials in our water ways. Is it safe?
Disclosure Statement
It is the policy of the Center for Continuing Education at Tulane University Health Sciences Center to ensure balance, independence, objectivity and scientific rigor in all its educational programs. All faculty participating in these programs are expected to disclose to the program audiences any real or apparent conflict of interest related to the content of their presentations. This information pertains to relationships with pharmaceutical companies, biomedical device manufacturers or other corporations whose products or services are related to the subject matter of the presentation topic or products in the research and development phase.